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Polycistronic Viruses

The polycistronic lentiviruses can deliver to the target cell all four transcription factors within a single expression cassette under the control of EF1α promoter. Each ORF is separated by unique 2A peptide (P2A, T2A, and E2A) sequences allowing simultaneous expression of all four transcription factors from a single vector. Such approach minimizes the number of proviral integrations required for successful cell reprogramming. This also reduces the risk of insertional mutagenesis in the induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses.

Vectors based on two protocols for both human and mouse sequences are available. Thomson's protocol (OSNL) includes Oct4, Sox2, Nanog or Lin28 genes, while Yamanaka's protocol (OSKM) includes Oct4, Sox2, KLF4 or Myc.

All viruses are VSV-G pseudotyped and capable of infecting both dividing and non-dividing cells. The expression of these transcription factors has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).

Select Product Cat.-# Unit Size Price, $ Quantity
Lenti-III-EF1α--mOSNL Virus
Mouse Oct4, Sox2, Nanog and Lin28
SCR-0057 200μl 1,950.00
Lenti-III-EF1α-hOSKM Virus
Human Oct4, Sox2, KLF4 and Myc
SCR-0056 200μl 1,950.00
Lenti-III-EF1α-hOSNL Virus
Human Oct4, Sox2, Nanog and Lin28
SCR-0055 200μl 1,950.00
Lenti-III-EF1α-mOSKM Virus
Mouse Oct4, Sox2, KLF4 and Myc
SCR-0058 200μl 1,950.00