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SubX® Plasma cfDNA Isolation kit

  • Cat.-#: CFDNA-0050
    Unit Size: 50 preps
    Price, $: 595.00
  • Qty:


SubX® Plasma cfDNA Isolation kit Description

cfDNA isolation from plasma, serum, and urine is based on binding the DNA to a dual-functional substance SubX® and adsorption onto a solid phase matrix under physiological conditions (e.g., directly from biological liquids). No sample dilution or Proteinase K digestion is required. The DNA isolation procedure involves three conventional steps:

1) DNA binding: simply add 15-50μl of SubX® to the sample, vortex and rotate for a few minutes, then add matrix slurry and rotate for 3-4 mniutes;

2) Washing: 3 brief vortex-spins;

3) Elution: DNA easily desorbed from matrix in a small volume (20-30μl) of elution buffer, which does not affect downstream applications.

Kit Content:

• SubX® Solution (14 ml). Ready-to-use. Store refrigerated +4C.
• Plasma Condition Solution (3 ml) Ready-to-use. Store at room temperature.
• Binding Matrix (1 ml). Ready-to-use. Store at room temperature in a dark place.
• Wash-1 Solution (30 ml). Ready-to-use. Store at room temperature in a dark place.
• Wash-2 Solution (2x11 ml). Add 55 ml of 96% molecular grade ethanol before use. Store at room temperature in a dark place.
• Elution Buffer: (5 ml); Ready-to-use. Store refrigerated +4C.

Kits are shipped at ambient temperatures.